mutagenesis in vitro meaning in Chinese
突变发生
Examples
- Cloning and mutagenesis in vitro of _ amylase gene were studied and four main contents included
本研究对-淀粉酶基因的克隆、诱变和表达等进行了系统的研究。 - We applied single cell gel electrophoresis and cell culture technique , which constitute sing cell gel electrophoresis assay system for detecting mutagenicity to detect mutagenesis in vitro induced by animal drug quinocetone and olaquindox . and confirmed optimum lysing - time . vero cells in the period of logarithm - growth time were treated with 9 . 1 ~ 273u mol / l h2o2 at 37 3h , then were lysed for lh , 2h , 3h and 4h to find optimum lysing - time
并基于阳性致突变物h _ 2o _ 2作用于非洲绿猴肾vero细胞引起细胞dna损伤的原理，研究了其关键步骤-裂解时间，以9 . 1 273 mol l剂量的h _ 2o _ 2染毒处于对数生长期的vero细胞3h后，收获细胞用于制备三明治凝胶板，分别裂解1h 、 2h 、 3h和4h并选择最适裂解时间。 - The new methods to identify _ amylase activity and its producing bacteria , and the preparation of high efficiency competence cell were reported . three _ amylase genes were cloned from bacillus subtilis , xanthomonas campestrisand aspergillus oryzae . two mutant genes with high activity was reported by using the methods of mutagenesis in vitro
主要研究内容包括-淀粉酶及其产生菌的快速鉴定新方法、高效感受态细胞的制备、 -淀粉酶基因的克隆和-淀粉酶基因的体外诱变等四个主要方面。